Who We Are
Our lab works on human genetics and the genetic basis for disease. We focus on inherited genetic instability/cancer predisposition syndromes including Werner and Bloom syndromes and Fanconi anemia, as well as on two specific types of cancer, adult acute myeloid leukemia (AML) and the gliomas.
Our research uses cell, molecular, computational and engineering approaches to address basic mechanistic questions and analyze clinical material, and to build useful pre-clinical disease models to enable translation. This work has been generously supported over the past decade by the NCI, NHLBI, the Bill and Melinda Gates Foundation/Foundation of the National Institutes of Health, the Fanconi Anemia Research Fund and the Butterfly Guild.
For more detail on specific projects, see our 'Projects' tab. To see what we've published from this work, see the 'Publications' tab.
2018 Mutagenesis Gordon Conference
Ray presented a summary of our TCGA DNA Damage and Repair project (see Knijnenburg et al. (2018) Cell Reports 23, 239–254 https://doi.org/10.1016/j.celrep.2018.03.076
2019 Target Malaria progress!
This is the year Target Malaria starts active fieldwork with our African partners, to use gene drive to control mosquito populations and suppress malaria transmission. Our Project Team Meeting in February was in Accra Ghana with colleagues from across Africa, and we had a follow-up in Seattle in September. For more detail see: https://targetmalaria.org/
Safe Harbors project gains traction!
Our Human Gene Therapy manuscript is out reporting a 10-fold increase in well-characterized human genomic 'safe harbor' sites (for details see: Human Gene Ther. 30:814-828. doi: 10.1089/hum.2018.169. A very useful toolkit for the well-characterized Chr4 SHS231 can be found at Addgene from this starter link: https://www.addgene.org/115143/.
2019 Fanconi Anemia Research Fund Symposium.
Three lab members, Tai Nguyen, Nyasha Chambwe and Ray participated in different ways in the Sept meeting in Chicago. Nyasha did a well-received talk on genetic variation in the Fanconi (FANC), ADH and ALDH gene families, while Tai presented a poster on our efforts to develop a cancer cell line resource.
Nyasha transitions to her new job at St. Jude as part of their cancer genomics team - go Nyasha! We miss you already!
Pellenz S, Phelps MP, Tang W, Hovde BT, Sinit R, Fu W, Li H, Chen E, and Monnat RJ Jr. (2019) New human chromosomal sage harbor sites for genome engineering with CRISPER/Cas9, TAL effector and homing endonucleases. Human Gene Therapy doi: 10.1089/hum.2018.169. [Epub ahead of print] and BiorXiv https://doi.org/10.1101/396390 (posted on 20 August 2018).
Juarez E, Chambwe N, Tang W, Mitchell AD, Owen N, Kumari A, Monnat RJ Jr, and McCullough AK. (2018) An RNAi screen in human cell lines reveals conserved DNA damage repair pathways that mitigate formaldehyde sensitivity. DNA Repair 72: 1-9. doi: 10.1016/j.dnarep.2018.10.002
Pathology and Genome Sciences
University of Washington
Seattle, WA 98195-7705
1959 N.E. Pacific Street
Room K-065 Health Sciences Bldg
Seattle, WA 98195-7705
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