Monnat Lab
2018 Publications
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Knijnenburg, T, Wang, L, Chambwe, N …Monnat, R.J., Jr., Xiao, Y. and Wang, C. on behalf of TCGA DNA Damage Repair Analysis Working Group (2018) Landscape of DNA damage repair deficiency across The Cancer Genome Atlas. Cell Rep. 2018 Apr; 23(1):239-254. https://doi.org/10.1016/j.celrep.2018.03.076 PMID: 29617664
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Oshima J., Fuki M. Hisama and Raymond J. Monnat, Jr. (2018) Werner Syndrome as a Model of Human Aging, chapter in Handbook of Models of Human Aging, 2nd Ed. P. Michael Conn, ed. Elsevier Academic Press (in press).
Lebel M, Monnat RJ Jr.. Werner syndrome (WRN) gene variants and their association with altered function and age-associated diseases. Ageing Res Rev. 2018 Jan;41:82-97. doi: 10.1016/j.arr.2017.11.003. Epub 2017 Nov 14. Review. PubMed PMID: 29146545.
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2019 Publications
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Pellenz S, Phelps MP, Tang W, Hovde BT, Sinit R, Fu W, Li H, Chen E, and Monnat RJ Jr. (2019) New human chromosomal sage harbor sites for genome engineering with CRISPER/Cas9, TAL effector and homing endonucleases. Human Gene Therapy doi: 10.1089/hum.2018.169. [Epub ahead of print] and BiorXiv https://doi.org/10.1101/396390 (posted on 20 August 2018).
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Juarez E, Chambwe N, Tang W, Mitchell AD, Owen N, Kumari A, Monnat RJ Jr, and McCullough AK. (2018) An RNAi screen in human cell lines reveals conserved DNA damage repair pathways that mitigate formaldehyde sensitivity. DNA Repair 72: 1-9. doi: 10.1016/j.dnarep.2018.10.002
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2017 Publications
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Kamath-Loeb AS, Zavala-van Rankin DG, Flores-Morales J, Emond MJ, Sidorova JM, Carnevale A, Cárdenas-Cortés MD, Norwood TH, Monnat RJ, Loeb LA, Mercado-Celis GE. Homozygosity for the WRN Helicase-Inactivating Variant, R834C, does not confer a Werner syndrome clinical phenotype. Sci Rep. 2017 Mar 9;7:44081. doi: 10.1038/srep44081. PMCID: PMC5343477.
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Fu W, Ligabue A, Rogers KJ, Akey JM, Monnat RJ Jr. Human RECQ Helicase Pathogenic Variants, Population Variation and "Missing" Diseases. Hum Mutat. 2017 Feb;38(2):193-203. doi: 10.1002/humu.23148. PMCID: PMC5518694.
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Oshima J, Sidorova JM, Monnat RJ Jr. Werner syndrome: Clinical features, pathogenesis and potential therapeutic interventions. Ageing Res Rev. 2017 Jan; 33:105-114. doi: 10.1016/j.arr.2016.03.002. PMCID: PMC5025328.
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Sidorova, Julia M, and Monnat Jr, Raymond J(Feb 2018) DNA Helicase Deficiency Disorders. In: eLS. John Wiley & Sons Ltd, Chichester. http://www.els.net [doi: 10.1002/9780470015902.a0006065.pub3]
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2016 Publications
Scharenberg, A.M., Stoddard, B.L. and Monnat, RJ Jr. (2016) Retargeting: an unrecognized consideration in endonuclease-based gene drive biology. bioRxiv record 089946; DOI: 10.1101/089946 (posted November 27, 2016).
Tokita M, Kennedy SR, Risques RA, Chun SG, Pritchard C, Oshima J, Liu Y, Bryant-Greenwood PK, Welcsh P, and Monnat RJ Jr. (2016) Werner syndrome through the lens of tissue and tumour genomics. Scientific Reports Aug 25;6:32038. PMID: 27559010. PMC4997333. DOI: 10.1038/srep32038.
Bosch LJW, Luo Y, Lao VV, Snaebjornsson P, Trooskens G, Vlassenbroeck I, Mongera S, Tang W, Welcsh P, Herman JG, Koopman M, Nagtegaal I, Punt CJA, van Criekinge W, Meijer G, Monnat, RJ Jr., Carvalho B, and Grady WM. (2016) WRN promoter CpG island hypermethylation does not predict more favorable outcomes for metastatic colorectal cancer patients treated with irinotecan-based therapy. Clinical Cancer Research Apr 27. Epub ahead of print. PMID: 27121793. PMC5026547. DOI: 10.1158/1078-0432.CCR-15-2703
Oshima J, Sidorova JM, and Monnat RJ Jr. (2016) Werner Syndrome. Aging Research Reviews Mar 15. pii: S1568-1637(16)30026-5. Epub ahead of print. Review. PMID: 26993153. PMC5025328 DOI: 10.1016/j.arr.2016.03.002
Zhang Q-S, Deater M, Phan N, Marcogliese AN, Tang W, Li H, Al-Dhalimy M, Major A, Monnat RJ Jr, Olson S, and Grompe M. (2016) Metformin alleviates hematopoietic defects and delays tumor formation in Fanconi anemia mice. Blood Oct 18. pii: Epub ahead of print. PMID:27756748. DOI:10.1182/blood-2015-11-683490
Kehrli K, Phelps M, Lazarchuk P, Chen E, Monnat RJ Jr. and Sidorova, JM. (2016) Class I histone deacetylase HDAC1 and WRN RECQ helicase contribute additively to protection of replication forks from hydroxyurea-induced arrest. J Biol Chem 291(47):24487-24503. Epub 2016 Sep 26. DOI:10.1074/jbc.M115.708594
Fu W, Ligabue A, Rogers KJ, Akey JM, and Monnat RJ Jr. (2016) Human RECQ helicase
pathogenic variants, population variation and 'missing' diseases. Human Mutation. Nov 17. doi: 10.1002/humu.23148. Epub ahead of print. PMID:27859906
Oshima J, Hisama FM, and Monnat RJ Jr. (2016) Werner Syndrome as a Model of Human Aging, chapter in Handbook of Models of Human Aging, 2nd Ed. P. Michael Conn, ed. Elsevier Academic Press (in press 20 Nov 2016).
Kamath-Loeb AS, Zavala-van Rankin DG, Flores-Morales J, Sidorova JM, Carnevale A, Cárdenas-Cortés M, Norwood TH, Monnat RJ Jr, Loeb LA, and Mercado-Celis GE. (2016) Marked gender distortion in homozygous carriers of the common, catalytically inactivating Werner syndrome R834C -WRN polymorphism. Scientific Reports (in revision Nov 2016).
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2015 Publications
Sidorova JM and Monnat RJ Jr. (2015) Human RECQ helicases: roles in cancer, aging, and inherited disease. Advances in Genomics and Genetics 5:19–33. DOI: 10.2147/AGG.S54078
Hovde BT, Deodato CR, Hunsperger HM, Ryken SA, Yost W, Jha RK, Patterson J, Monnat RJ Jr, Barlow SB, Starkenburg SR, Cattolico RA. (2015) Genome sequence and transcriptome analyses of Chrysochromulina tobin: metabolic tools for enhanced algal fitness in the prominent order Prymnesiales (Haptophyceae). PLoS Genetics 11(9):e1005469. PMID: 26397803. PMC4580454. DOI: 10.1371/journal.pgen.1005469.
Tang W, Robles AI, Beyer RP, Gray LT, Nguyen GH, Oshima J, Maizels N, Harris CC, and Monnat RJ Jr. (2015) Werner syndrome helicase modulates G4 DNA-dependent transcription and opposes mechanistically distinct senescence-associated gene expression programs. bioXriv Biology Preprint server, published as New Results on 4 August 2015 under bioXriv DOI: 10.1101/023739
Tang W, Robles AI, Beyer RP, Gray LT, Nguyen GH, Oshima J, Maizels N, Harris CC, and Monnat RJ Jr. (2015) Werner syndrome helicase targets G4 DNA in human cells to modulate transcription and suppress genomic instability. Human Molecular Genetics, Mar 16. Epub ahead of print. PMID: 26984941, PMC5062591 DOI: 10.1093/hmg/ddw079
Robinson R, Wonga L, Monnat RJ Jr., and Fu E. (2015) Development of a whole blood paper-based device for phenylalanine detection and PKU therapy monitoring. Micromachines 7(2), 28; DOI: 10.3390/mi7020028.
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2014 Publications
Lauper JM, Monnat RJ Jr. (2014) Diabetes mellitus and cancer in Werner syndrome. Acta Diabetol. 51:159-61. doi: 10.1007/s00592-013-0456-z. PMCID: PMC3669637.
Friedman, J.I. Li, H. and Monnat, RJ Jr. (2014) Quantifying the information content of homing endonuclease target sites by single base pair profiling. Chapter 11 in David R. Edgell (ed.), Homing Endonucleases: Methods and Protocols, Methods in Molecular Biology, vol. 1123. DOI 10.1007/978-1-62703-968-0_11.
Li, H. and Monnat, RJ Jr. (2014) Homing endonuclease target site specificity defined by sequential enrichment and next-generation sequencing of highly complex target site libraries. Chapter 12 in David R. Edgell (ed.), Homing Endonucleases: Methods and Protocols, Methods in Molecular Biology, vol. 1123. DOI 10.1007/978-1-62703-968-0_12.
Pellenz, S. and Monnat, RJ Jr. (2014) Identification and analysis of genomic homing endonuclease target sites. Chapter 16 in David R. Edgell (ed.), Homing Endonucleases: Methods and Protocols, Methods in Molecular Biology, vol. 1123. DOI 10.1007/978-1-62703-968-0_16.
Shiovitz S, Bertagnolli MM, Renfro LA, Nam E, Foster NR, Dzieciatkowski S, Valinluck-Lao V, Monnat RJ Jr, Emond M, Maizels N, Niedzwiecki D, Saltz LB, Venook A, Warren RS, Grady WM. (2014) CpG island methylator phenotype predicts improved response to adjuvant therapy with irinotecan, fluorouracil, and leucovorin in stage III colon cancer: Cancer and Leukemia Group B Protocol 89803. Gastroenterology 147(3):637-45. doi: 10.1053/j.gastro.2014.05.009. PMCID: PMC4143495.
Nguyen, G.H., Tang,W., Robles, A.I., Beyer, R.P., Gray, L.T., Welsh, J., Kumamoto, K. Wang, X.W., Hickson, I.D., Maizels, N., Monnat, R.J. Jr. and Harris, C.C. (2014) Regulation of gene expression by the BLM helicase correlates with the presence of G-quadruplex DNA motifs. Proc Natl Acad Sci U S A. 111: 9905-9910. doi: 10.1073/pnas.1404807111. PMCID: PMC4103369.
Hovde, B.T., Starkenburg, S.R., Hunsperger, H.M., Mercer, L.D., Deodato, C. Jha, R.K., Chertkov, O., Monnat, R.J., Jr. and Cattolico, R.A. (2014) The mitochondrial and chloroplast genomes of the haptophyte Chrysochromulina tobin contain unique repeat structures and gene profiles. BMC Genomics 15(1):604. doi: 10.1186/1471-2164-15-604. PMID: 25034814
Rogers, K.J., Fu, W., Akey, J.M. and Monnat, R.J., Jr. (2014) Global and disease-associated genetic variation in the human Fanconi anemia gene family. Human Molecular Genetics 23:6815-25. doi: 10.1093/hmg/ddu400 PMCID: PMC4245043.
Pavelitz T, Renfro L, Foster NR, Caracol A, Welsch P, Lao VL, Grady WM, Niedzwicki D, Saltz LB, Bertagnolli M, Goldberg RM, Rabinovitch PS, Emond M, Monnat RJ Jr, Maizels N. (2014) MRE11-Deficiency predicts improved long-term disease free survival and overall survival in a subset of stage III colon cancer patients treated with irinotecan: Cancer and Leukemia Group B Protocol 89803. PLoS ONE 9(10):e108483. doi: 10.1371/journal.pone.0108483. PMCID: PMC4195600.
Chang, TC, Mikheev, A.M., Huynh, W., Monnat, R.J., Jr., Rostomily, R.C. and Folch, A. (2014) Parallel microfluidic chemosensitivity testing on individual slice cultures. Lab Chip 14:4540-51. doi: 10.1039/c4lc00642a. PMCID: PMC4217250.
Thiessen G, De Los Reyes K, Monnat, R.J., Jr. and Fu E (2014) An equipment-free paper-based test for the detection of phenylketonuria in newborns in low-resource settings. The Analyst 140:609-15. doi: 10.1039/c4an01627k. PMID: 25427275 [PubMed - in process].
Chang, C., Tang, W., Koh, W.J.H., Emond, M.J., Monnat, R.J., Jr. and Folch, A. (2014) Microwell arrays reveal cellular heterogeneity during the clonal expansion of transformed human cells. Technology 3: 163-171. PMC4854201. doi: 10.1142/S2339547815200046.
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2013 Publications
Sidorova JM, Kehrli K, Mao F, Monnat R Jr. (2013) Distinct functions of human RECQ helicases WRN and BLM in replication fork recovery and progression after hydroxyurea-induced stalling. DNA Repair 12:128-39. doi: 10.1016/j.dnarep.2012.11.005. PMCID: PMC3551992
Lauper JM, Monnat RJ Jr. (2013) Diabetes mellitus and cancer in Werner syndrome. Acta Diabetol. 51:159-61. doi: 10.1007/s00592-013-0456-z. PMCID: PMC3669637
Monnat, R.J., Jr. (2013) ‘Werner syndrome’, in WHO/IARC Monograph on Pathology and Genetics of Tumours of Soft Tissue and Bone, 4th Edition edited by C.D.M. Fletcher, J.A. Bridge, P.C.W. Hogendoorn and F.Mertens. IARC Press, Lyon. pp. 393-394.
Berti M, Chaudhuri AR, Thangavel S, Gomathinayagam S, Kenig S, Vujanovic M, Odreman F, Glatter T, Graziano S, Mendoza-Maldonado R, Marino F, Lucic B, Biasin V, Gstaiger M, Aebersold R, Sidorova JM, Monnat RJ Jr, Lopes M, Vindigni A. (2013) Human RECQ1 promotes restart of replication forks reversed by DNA topoisomerase I inhibition. Nature Structural Molec Biol. 20:347-54. doi: 10.1038/nsmb.2501. PMCID: PMC3897332
Lauper, J.L. Krause, A., Vaughan, T.L. and Monnat, R.J.,Jr. (2013) Spectrum and risk of neoplasia in Werner syndrome: a systematic review. PLoS ONE 8(4):e59709. doi: 10.1371/journal.pone.0059709. PMCID: PMC3613408
Glukhova VA, Tomazela DM, Findlay GD, Monnat RJ Jr, Maccoss MJ. (2013) Rapid Assessment of RNAi-mediated Protein Depletion by Selected Reaction Monitoring Mass Spectrometry. J Proteome Res. 12(7):3246-54. DOI: 10.1021/pr400067k. PMCID: PMC3767422
Metzger MJ, Stoddard BL, Monnat RJ Jr. (2013) PARP-mediated repair, homologous recombination, and back-up non-homologous end joining-like repair of single-strand nicks. DNA Repair 12:529-34. doi: 10.1016/j.dnarep.2013.04.004. PMCID: PMC3683368
Hughes BT, Sidorova J, Swanger J, Monnat RJ Jr, Clurman BE. (2013) Essential role for Cdk2 inhibitory phosphorylation during replication stress revealed by a human Cdk2 knockin mutation. Proc Natl Acad Sci U S A. 110:8954-9. doi: 10.1073/pnas.1302927110. PMCID: PMC3670391
Lao VV, Welcsh P, Luo Y, Carter KT, Dzieciatkowski S, Dintzis S, Meza J, Sarvetnick NE, Monnat RJ Jr, Loeb LA and Grady WM (2013) Altered RECQ helicase expression in sporadic primary colorectal cancers. Transl Oncol. 6:458-69. doi:10.1593/tlo.13238. PMCID: PMC3730021.
2012 Publications
Duxin JP, Moore HR, Sidorova J. Karanja, K, Honaker Y, Dao B, Piwnica-Worms H, Campbell JL, Monnat RJ Jr, Stewart SA (2012) An Okazaki fragment processing-independent role for human Dna2 during DNA replication. J. Biol. Chem. 287:21980-91. doi: 10.1074/jbc.M112.359018. PMCID: PMC3381158
Li H, Ulge UY, Hovde BT, Doyle LA, Monnat RJ Jr. (2012) Comprehensive homing endonuclease target site specificity profiling reveals evolutionary constraints and enables genome engineering applications. Nucleic Acids Res. 40(6):2587-98. doi: 10.1093/nar/gkr1072. PMCID: PMC3315327
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2010 Publications
Dhillon,K.K., Sidorova,J.M., Albertson,T.M., Anderson,J.B., Ladiges,W.C., Rabinovitch,P.S., Preston, B.D. and Monnat, R.J., Jr. (2010) Werner syndrome protein loss leads to divergent mouse and human cell phenotypes. DNA Repair 9:11-22. doi: 10.1016/j.dnarep.2009.09.013. PMCID: PMC2818259
Bhavan-Thangavel, S., Mendoza-Maldonado, R. Tissino, E. Sidorova, J.M., Yin,J. Wang, W., Monnat, R.J., Jr., Falaschi, A. and Vindigni, A. (2010) The human RECQ1 and RECQ4 helicases play distinct roles in DNA replication initiation. Molec Cell Biol. 30:1382-1396 (on-line 11 Jan 2010; doi:10.1128/MCB.01290-09). PMCID: PMC2832491
Mao, F.J., Sidorova, J.M., Lauper, J.M., Emond, M.E. and Monnat, R.J., Jr. (2010) The human WRN and BLM RecQ helicases differentially regulate cell proliferation and survival after chemotherapeutic DNA damage. Cancer Research 70(16):6548-55. Epub 2010 Jul 27. doi: 10.1158/0008-5472.CAN-10-0475. PMCID: PMC2941797
Sidorova, J.M. and Monnat, R.J., Jr. (2010) DNA Helicase-deficiency Disorders. Encyclopedia of Life Sciences, Article # A6065. DOI: 10.1002/9780470015902.a0006065.pub2
Monnat, R.J., Jr. (2010) Human RECQ helicases: Roles in DNA metabolism, disease pathogenesis and disease therapy. Seminars Cancer Biol. 20(5):329-39. doi: 10.1016/j.semcancer.2010.10.002. PMCID: PMC3040982
Ulge, U.Y., Baker, D.A. and Monnat, R.J., Jr. (2010) Comprehensive computational design of mCreI homing endonuclease cleavage specificity for genome engineering. Nucleic Acids Research 39(10):4330-9. doi: 10.1093/nar/gkr022. PMCID: PMC3105429
Windbichler, N. Menichelli, M., Papathanos, P.A., Thyme, S.B., Li, H., Ulge, U.Y. Hovde, B.T., Baker, D., Monnat, R.J., Jr., Burt, A. and Crisanti, A. (2010) A synthetic, homing ednouclease- based genetic drive system in the human malaria mosquito. Nature 473(7346):212-5. doi:10.1038/nature09937. PMCID: PMC3093433
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2009 Publications
McConnell-Smith, A., Pellenz, S., Davis, L.W., Maizels, N., Monnat, R.J., Jr. and Stoddard, B.L. (2009) Generation of a nicking enzyme that stimulates site-specific targeted gene correction from the I-AniI LAGLIDADG homing endonuclease. Proc Natl Acad Sci USA 106:5099-5104. doi: 10.1073/pnas.0810588106. PMCID: PMC2664052
Li, H. Pellenz, S., Ulge, U. Stoddard, B.L. and Monnat, R.J., Jr. (2009) Generation of single-chain LAGLIDADG homing endonucleases from native homodimeric precursor proteins. Nucleic Acids Res. 37:1650-1662. doi: 10.1093/nar/gkp004. PMCID: PMC2655683
Sidorova, J.M., Li, N., Schwartz, D.C., Folch, A. and Monnat, R.J., Jr. (2009) Microfluidic-assisted analysis of replicating DNA molecules. Nature Protocols 4:849-861. doi: 10.1038/nprot.2009.54. PMCID: PMC2762429
Rey, L., Sidorova, J.M., Puget, N., Boudsocq, F., Biard, D.S.F., Monnat, R.J., Jr., Cazaux, C. and Hoffman, J-S. (2009) Human DNA polymerase eta is required for common fragile site stability during unperturbed DNA replication. Molec Cell Biol 29:3344-3354. doi: 10.1128/MCB.00115-09. PMCID: PMC2698728
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2008 Publications
Sidorova, J.M., Li, N. Folch, A. and Monnat, R.J., Jr. (2008) The Werner syndrome RecQ helicase is required for normal replication fork progression after DNA damage or replication arrest. Cell Cycle 7:796-807. PMCID: PMC4362724
Stoddard, B.L. Scharenberg, A.M. and Monnat, R.J., Jr. (2008) Advances in engineering homing endonucleases for gene targeting: Ten years after structures. Chapter 6 in Progress in Gene Therapy 3: Autologous and Cancer Stem Cell Gene Therapy, Eds. R. Bertolotti and K. Ozawa, World Scientific Press, Hackensack, NJ. pp. 135-167. DOI: 10.1142/9789812775870_0006
Berkovich, E., Monnat, R.J., Jr. and Kastan, M.B. (2008) Assessment of protein dynamics and DNA repair following generation of DNA double-strand breaks at defined genomic sites. Nature Protocols 3:915-922. doi: 10.1038/nprot.2008.54. PMID: 18451799
Loeb,L.A. and Monnat, R.J., Jr. (2008) DNA polymerases and human disease. Nature Reviews Genetics 9: 594-604. doi: 10.1038/nrg2345. PMID: 18626473
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2007 Publications
Dhillon,K., Sirorova, J., Saintigny,Y., Poot,M., Gollahon,K., Rabinovitch, P.S. and Monnat, R.J.,Jr. (2007) Functional role of the Werner syndrome RecQ helicase in human fibroblasts. Aging Cell 6: 53-61. DOI: 10.1111/j.1474-9726.2006.00260.x. PMID: 17266675
Sharma A, Awasthi S, Harrod CK, Matlock EF, Khan S, Xu L, Chan S, Yang H, Thammavaram CK, Rasor RA, Burns DK, Skiest DJ, Van Lint C, Girard AM, McGee M, Monnat RJ Jr, Harrod R. (2007) The Werner syndrome helicase is a cofactor for HIV-1 long terminal repeat transactivation and retroviral replication. J Biol Chem. 282:12048-57. doi: 10.1074/jbc.M608104200. PMID: 17317667
Volna, P., Jarjour, J. Baxter, S., Roffler, S., Monnat, RJ Jr., Stoddard, B. and Scharenberg, A. (2007) Flow cytometric analysis of DNA binding and cleavage by cell surface-displayed homing endonucleases. Nucleic Acids Research 35: 2748-2758. doi: 10.1093/nar/gkm182. PMCID: PMC1885675
Berkovich, E., Monnat, RJ Jr. and Kastan, M. (2007) Roles of ATM and NBS1 in chromatin structure modulation and DNA double strand break repair. Nature Cell Biology 9: 683-690. doi:10.1038/ncb1599. PMID: 17486112
Monnat, RJ Jr. (2007) From broken to old: DNA damage, IGF1 endocrine suppression and aging. DNA Repair 6: 1386-1390 (invited commentary). doi: 10.1016/j.dnarep.2007.03.018. PMCID: PMC2704237
Eastberg, J., Eklund, J., Monnat, RJ Jr. and Stoddard, B. (2007) Mutability of an HNH nuclease imidazole general base and exchange of a deprotonation mechanism. Biochemistry 46: 7215- 7225. DOI: 10.1021/bi700418d. PMID: 17516660
Kudlow, B., Kennedy, B.K., and Monnat, RJ Jr. (2007) Werner and Hutchinson-Gilford progeria syndromes: mechanistic basis of human progeroid diseases. Nature Reviews Molecular and Cellular Biology 8: 394-404. doi:10.1038/nrm2161. PMID: 17450177
Eklund, J.L., Ulge, U.Y., Eastberg, J. and Monnat, R.J., Jr. (2007) Altered target site specificity variants of the I-PpoI His-Cys box homing endonuclease. Nucleic Acids Research 35:5839- 5850. doi: 10.1093/nar/gkm624. PMCID: PMC2034468
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2006 Publications
Monnat, R.J., Jr. (2006) Werner Syndrome as a Model of Human Aging. Handbook of Models of Human Aging, P. Michael Conn, ed. Elsevier Academic Press, Chapter 80, pp. 961-976.
Ashworth, J., Havranek, J.J., Duarte, C.M., Sussman, D., Monnat, R.J., Jr., Stoddard, B.L. and Baker, D. (2006) Computational redesign of endonuclease DNA binding and cleavage specificity. Nature 441:656-659. doi:10.1038/nature04818.
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2005 Publications
Awasthi S, Sharma A, Wong K, Zhang J, Matlock EF, Rogers L, Motloch P, Takemoto S, Taguchi, H, Cole MD, Luscher B, Dittrich O, Tagami H, Nakatani Y, McGee M, Girard AM, Gaughan L, Robson CN, Monnat RJ Jr, and Harrod R.(2005) A human T-cell lymphotropic virus type 1 enhancer of Myc transforming potential stabilizes Myc-TIP60 transcriptional interactions. Mol Cell Biol. 25:6178-98. doi: 10.1128/MCB.25.14.6178-6198.2005. PMCID: PMC1168837
Chevalier, B., Monnat, R.J., Jr. and Stoddard, B.L. (2005) The LAGLIDADG Homing Endonuclease Family. Homing Endonucleases and Inteins, M. Belfort, B.L. Stoddard, D.W. Wood, V. Derbyshire (Eds). Springer, Berlin. pp.33-47. Doi: 10.1007/3-540-29474-0_3
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2004 Publications
Swanson, C., Saintigny, Y., Emond, M.J. and Monnat, R.J., Jr. (2004) The Werner syndrome protein has separable recombination and survival functions. DNA Repair 3:475-482. doi:10.1016/j.dnarep.2004.01.002. PMID: 15084309
Monnat, R.J., Jr. and Saintigny, Y. (2004) The Werner syndrome protein: in vivo function and disease links. Science SAGE-KE (sageke.sciencemag.org/cgi/content/full/sageke;2004/13/re3). DOI: 10.1126/sageke.2004.13.re3.
Sussman, D., Chadsey, M., Fauce, S., Engel, A., Bruett, A., Monnat, R. Jr., Stoddard, B. L. and Seligman, L.M. (2004) Isolation and characterization of new homing endonuclease specificities at individual target site positions. J. Mol. Biol. 342:31-41. doi:10.1016/j.jmb.2004.07.031. PMID: 15313605
Chevalier, B.S., Sussman, J., Otis, C., Nöel, A-J., Turmel, M., Lemieux, C., Stephens, K., Monnat,R.J., Jr. and Stoddard, B.L.(2004) Metal-dependent DNA cleavage mechanism of the I- CreI LAGLIDADG homing endonuclease. Biochemistry 43:14015-14022. DOI: 10.1021/bi048970c. PMID: 15518550
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2003 Publications
Chevalier, B.S., Turmel, M., Lemieux, C., Monnat, R.J., Jr. and Stoddard, B.L.(2003) Flexible DNA target site recognition by divergent homing endonuclease isoschizomers I-CreI and I-MsoI. J. Mol. Biol. 329:253-269. Doi: 10.1016/S0022-2836(03)00447-9. PMID: 12758074
Grandori, C., Wu, K-J., Fernandez, P., Ngouenet, C., Grim, J., Clurman, B.E., Moser, M.J., Oshima,J., Russell, D.W., Swisshelm, K., Frank, S., Amati, B., Dalla-Favera, R. and Monnat, R.J., Jr. (2003) Werner syndrome protein limits MYC-induced cellular senescence. Genes & Development 17:1569-1574. doi: 10.1101/gad.1100303. PMCID: PMC196129
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2002 Publications
Saintigny, Y. and Monnat, R.J., Jr. (2002) L’Hélicase RecQ, dont les altérations sont à l’origine du syndrome de Werner, participe à la réparation de l’ADN par recombinaison homologue. Médecine Sciences 18:79-85. doi: 10.1051/medsci/200218179.
Colgin, L.M., Hackmann, A.F.M., Emond, M.J. and Monnat, R.J., Jr. (2002) The unexpected landscape of in vivo somatic mutation in a human epithelial cell lineage. Proc. Natl. Acad. Sci. USA 99:1437-1442. doi: 10.1073/pnas.032655699. PMCID: PMC122209
Saintigny,Y., Makienko, K., Swanson, C., Emond, M.J. and Monnat, R.J., Jr. (2002) A homologous recombination resolution defect in Werner syndrome. Molec. Cell. Biol. 22:6971- 6978. doi: 10.1128/MCB.22.20.6971-6978.2002. PMCID: PMC139822
Chevalier, B.S., Kortemme,T., Chadsey, M.S., Baker, D., Monnat, R.J., Jr. and Stoddard, B.L. (2002) Design, activity and structure of E-DreI, a highly site-specific artificial endonuclease. Molecular Cell 10:895-905. DOI: http://dx.doi.org/10.1016/S1097-2765(02)00690-1. PMID: 12419232
Monnat, R.J., Jr. (2002) Werner syndrome. WHO/IARC Monograph on Pathology and Genetics of Tumours of Soft Tissue and Bone, edited by C. Fletcher, K. Unni and F.Mertens. IARC Press, Lyon. pp. 366-367.
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2001 Publications
Chevalier, B.S., Monnat, R.J., Jr. and Stoddard, B.L. (2001) The LAGLIDADG homing endonuclease I-CreI shares three metals between two active sites. Nature Structural Biology 8:312-316. doi:10.1038/87656
Prince, P.R., Emond, M.J. and Monnat, R.J., Jr. (2001) Loss of Werner syndrome protein function promotes aberrant mitotic recombination. Genes & Development 15:933-938. doi: 10.1101/gad.877001. PMCID: PMC312674
Monnat, R.J., Jr. (2001) Cancer pathogenesis in the human RecQ helicase deficiency syndromes. From Premature Gray Hair to Helicase: Werner Syndrome Implications for Aging and Cancer. Edited by M. Goto and R.W. Miller. GANN Monograph on Cancer Research 49:83-94.
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2000 Publications
Moser, M.J., Kamath-Loeb, A.S., Jacob, J.E., Bennett, S.E., Oshima, J. and Monnat, R.J., Jr. (2000) WRN helicase expression in Werner syndrome cell lines. Nucleic Acids Res. 28:648-654. doi: 10.1093/nar/28.2.648. PMCID: PMC102521
Moser, M.J., Bigbee, W.L., Grant, S.G., Emond, M.J., Langlois, R.G., Jensen, R.H., Oshima, J. and Monnat,R.J., Jr. (2000) Genetic instability and hematologic disease risk in Werner syndrome patients and heterozygotes. Cancer Research 60:2492-2496. PMID: 10811130
Flory, M.R., Moser, M.J., Monnat, R.J., Jr. and Davis, T.N. (2000) Identification of a human centrosomal calmodulin-binding protein that shares homology with pericentrin. Proc. Natl. Acad. Sci. USA 97:5919-5923. PMCID: PMC18534
Galburt, E.A., Chadsey, M.S., Jurica, M.S., Chevalier, B.S., Ehro, D., Tang, W., Monnat, R.J., Jr. and Stoddard, B.L. (2000) Conformational changes and cleavage by the homing endonuclease I-PpoI: a critical role for a leucine residue in the active site. J. Mol. Biol. 300:877-887. doi:10.1006/jmbi.2000.3874. PMID: 10891275
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1999 Publications
Bennett, S.E., Umar, A., Kodama, S., Barrett, J.C., Monnat, R.J., Jr. and Kunkel, T.A. (1999) Evidence against a role for the Werner syndrome gene product in DNA mismatch repair. Molecular biology of Aging, Alfred Benzon Symposium Proceedings, No. 44. Bohr, V.A., Clark, B.F.C. and Stevnsner, T. Munksgaard, Copenhagen. pp.214-224.
Moser, M.J., Oshima, J., and Monnat, R.J., Jr. (1999) Invited Mutation Update: WRN mutations in Werner syndrome. Human Mutation 13:271-279.
Monnat, R.J., Jr., Hackmann, A.F.M. and Cantrell, M.A. (1999) Generation of highly site-specific DNA double strand breaks in human cells by the homing endonucleases I-PpoI and I-CreI. Biochem. Biophys. Res. Comm. 255:88-93. Article id: bbrc.1999.0152 PMID: 10082660
Colgin, L.M., Hackmann, A.F.M., and Monnat, R.J., Jr. (1999) Different somatic and germline HPRT1 mutations promote use of a common, cryptic intron 1 splice site. Human Mutation 13:504-505 (also published electronically as Human Mutation Mutation in Brief #246 (1999) Online: http://journals.wiley.com/1059-7794/pdf/mutation/246.pdf DOI: 10.1002/(SICI)1098- 1004(1999)14:2<182::AID-HUMU12>3.0.CO;2-Y. PMID:10447274
Prince, P.R., Ogburn, C.E., Moser, M.J., Emond, M.J., Martin, G.M. and Monnat, R.J., Jr. (1999) Cell fusion corrects the 4-nitroquinoline 1-oxide sensitivity of Werner syndrome fibroblast cell lines. Human Genetics 105:132-138. PMID: 10480367
Monnat, R.J., Jr. (1999) Unusual features of thyroid carcinomas in Japanese patients with Werner syndrome and possible genotype-phenotype relations to cell type and race (originally ‘No association of specific WRN mutations with thyroid cancer risk or histologic subtype in Werner syndrome patients’ prior to editorial retitling). Cancer 86:728-729.
Galburt, E., Chevalier, B., Tang, W., Jurica, M.S., Flick, K.E., Monnat, R.J., Jr. and Stoddard,B.L. (1999) A novel endonuclease mechanism directly visualized for I-PpoI. Nature Structural Biology 6:1096-1099. doi:10.1038/70027. PMID: 10581547
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1998 Publications
Argast, G., Stephens, K.M., Emond, M.J., and Monnat, R.J., Jr. (1998) I-PpoI and I- CreI homing site sequence degeneracy determined by random mutagenesis and sequential in vitro enrichment. J. Mol. Biol. 280:345-353. doi:10.1006/jmbi.1998.1886. PMID: 9665841
Flick, K.E., Jurica, M.S., Monnat, R.J., Jr. and Stoddard, B.L. (1998) DNA binding and cleavage by the nuclear intron-encoded homing endonuclease I-PpoI. Nature 394:96-101. doi:10.1038/27952. PMID: 9665136
Jurica, M.S., Monnat, R.J., Jr. and Stoddard, B.L. (1998) DNA recognition and cleavage by the LAGLIDADG homing endonuclease I-CreI. Molecular Cell 2:469-476. doi:10.1016/S1097-2765(00)80146-X. PMID: 9809068
Moser, M.J., Oshima, J., Martin, G.M. and Monnat, R.J.,Jr. (1998) Werner syndrome mutational database and website. Available at: http://www.pathology.washington.edu/werner/ (1 November 1998).
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1997 Publications
Brooks-Wilson, A.R., Emond, M. and Monnat, R.J., Jr. (1997) Paradoxically low loss of heterozygosity (LOH) in genetically unstable Werner syndrome cell lines. Genes, Chromosomes & Cancer 18:133-142.
Stephens, K.M., Monnat, R.J., Jr., Heath, P.D., and Stoddard, B.M. (1997) Crystallization and preliminary X-ray analysis of the Chlamydomonas reinhardtii I-CreI mobile intron endonuclease. Proteins: Structure, Function and Genetics 28:137-139. DOI: 10.1002/(SICI)1097-0134(199705)28:1<137::AID-PROT15>3.0.CO;2-K
Heath, P.D., Stephens, K.M., Monnat, R.J., Jr. and Stoddard, B.M. (1997) The structure of I-CreI: a Group I intron-encoded homing endonuclease. Nature Structural Biology 4:468-476. doi:10.1038/nsb0697-468.
Bennett, S., Umar, A., Oshima, J., Monnat, R.J., Jr. and Kunkel, T.M. (1997) Mismatch repair in extracts from Werner syndrome cell lines. Cancer Research 57: 256-2960. PMID: 9230208
Seligman, L.M., Stephens, K.M. and Monnat, R.J., Jr. (1997) Genetic analysis of the Chlamydomonas reinhardtii I-CreI mobile intron homing system in Escherichia coli. Genetics 147:1653-1664. PMCID: PMC1208338
Flick, K.E., McHugh, D., Heath, J.D., Stephens, K.M., Monnat, R.J., Jr., and Stoddard, B.L. (1997) Crystallization and preliminary X-ray studies of I-PpoI: a nuclear, intron-encoded homing endonuclease from P. polycephalum. Protein Science 6:1-4. DOI: 10.1002/pro.5560061226. PMCID: PMC2143617
Bridges, B.A., Drake, J.A., Fishel, R., Foster, P., Monnat, R.J., Jr., Ripley, L.S. and Taddei, F. (1997). “Optimum mutation rates in evolution and disease” (invited and moderated on-line discussion as part of the HMS Beagle Cutting Edge Debate series). HMS Beagle (13), 25 July 1997 (HMS Beagle web site: http://biomednet.com/hmsbeagle/14/cutedge/ overview.htm).
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1996 Publications
Martin, G.M., Ogburn, C.E., Colgin, L.M., Gown, A.M., Edland, S.D. and Monnat, R.J., Jr. (1996) Somatic mutations are frequent and increase with age in human kidney epithelial cells. Human Molec. Genetics 5:215-221. doi: 10.1093/hmg/5.2.215. PMID: 8824877
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1993 Publications
Bendich,A.J., Loretz, C.J., and Monnat, R.J.,Jr. (1993) The structure of the plant mitochondrial genome. Plant Mitochondria: with emphasis on RNA editing and cytoplasmic male sterility, ed. A. Brennicke and U. Kück, pp.171-180. VCH Publishers, Inc, Weinheim.
Liu, P.K., Trujillo, J.M., and Monnat, R.J., Jr. (1993) Spectrum of spontaneous mutation in animal cells containing an aphidicolin-resistant DNA polymerase α. Mutation Research 288:229-236. doi:10.1016/0027-5107(93)90089-X. PMID: 7688082
Maresh, G.A. and Monnat, R.J., Jr. (1993) Novel fluoride-stimulated dichlorofluorescein dye oxidation pathway in human leukemia cell lines. Biochem Biophys Res. Comm. 194:869-875. doi:10.1006/bbrc.1993.1902. PMID: 8393671
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1992 Publications
Chiaverotti, T.A, Battula, N., and Monnat, R.J., Jr. (1992) Rat hypoxanthine phosphoribo-syltransferase cDNA cloning and sequence analysis. Genomics 11:1158-1160. doi:10.1016/0888-7543(91)90046-H. PMID: 1783384
Monnat, R.J., Jr., Hackmann, A.F.M., and Chiaverotti, T.A. (1992) Nucleotide sequence analysis of human hypoxanthine phosphoribosyltransferase (HPRT) gene deletions. Genomics 11:777-787. doi:10.1016/0888-7543(92)90153-J. PMID: 1639404
Monnat, R.J., Jr., Chiaverotti, T.A., Hackmann, A.F.M., and Maresh, G.A. (1992) Molecular structure and genetic stability of human hypoxanthine phosphoribosyltransferase (HPRT) gene duplications. Genomics 11:788-796. doi:10.1016/0888-7543(92)90154-K. PMID: 1639405
Monnat, R.J., Jr. (1992) Werner syndrome: molecular genetics and mechanistic hypotheses. Exp. Gerontol. 27:447-453. PMID: 1459221
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1991 Publications
Monnat, R.J., Jr., Hackmann, A.F.M., Chiaverotti, T.A., and Maresh, G.A. (1991) Molecular analysis of human hprt gene deletions and duplications. Adv. Exp. Biol. Med. 309B:113-116. PMID: 1781354
Chiaverotti, T.A., Battula, N. and Monnat, R.J., Jr. (1991) Rat hypoxanthine phosphoribosyltransferase cDNA cloning and sequence analysis. Adv. Exp. Biol. Med. 309B:117-120. PMID:
1781355
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1990 Publications
Fukuchi, K.-i., Tanaka, K., Kumahara, Y., Marumo, K., Pride, M.B., Martin, G.M., and Monnat,R.J., Jr. (1990) Increased frequency of 6-thioguanine-resistant peripheral blood lymphocytes in Werner syndrome patients. Human Genetics 84:249-252. PMID:2303247
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1989 Publications
Monnat, R.J., Jr. (1989) Molecular analysis of spontaneous hypoxanthine phosphoribosyl-transferase mutations in thioguanine-resistant HL-60 human leukemia cells. Cancer Research 49:81-87. PMID: 2908855
Fukuchi, K.-i., Martin, G.M., and Monnat, R.J., Jr. (1989) Mutator phenotype of Werner syndrome is characterized by extensive deletions. Proc. Natl. Acad. Sci. USA 86:5893-5897 (errata in Proc. Natl. Acad. Sci. USA 86:7994, 1989). PMCID: PMC297737
Monnat, R.J., Jr. (1989) General pathology teaching at the University of Washington. J. Pathol. 159:345-349. PMID: 2614580
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1988 Publications
Mita, S., Monnat, R.J., Jr. and Loeb, L.A. (1988) Direct selection of mutations in the human mitochondrial tRNAThr gene: reversion of an "uncloneable" phenotype. Mutation Research 199:183-190. doi:10.1016/0027-5107(88)90244-8. PMID: 3362158
Turker, M.S., Monnat, R.J., Jr., Fukuchi, K., Johnston, P.A., Ogburn, C.E., Weller, R.E., Park, J.F. and Martin, G.M. (1988) A novel class of unstable 6-thioguanine resistant cells from dog and human kidneys. Cell Biol.Toxicol. 4:211-223. PMID: 3233532
Mita, S., Monnat, R.J., Jr. and Loeb, L.A. (1988) Resistance of HeLa cell mitochondrial DNA to mutagenesis by chemical carcinogens. Cancer Research 48:4578-4583. PMID: 3135115
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1986 Publications
Monnat, R.J., Jr. and Reay, D.T. (1986) Nucleotide sequence identity of mitochondrial DNA from different human tissues. Gene 43:205-211. doi:10.1016/0378-1119(86)90208-8. PMID: 3744049
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1985 Publications
Monnat, R.J., Jr. and Loeb, L.A. (1985) Nucleotide sequence preservation of human mitochondrial DNA. Proc. Natl. Acad. Sci. USA 82:2895-2899. PMID: 2986144
Monnat, R.J., Jr., Maxwell, C.L. and Loeb, L.A. (1985) Nucleotide sequence preservation of human leukemic mitochondrial DNA. Cancer Research 45:1809-1814. PMID: 3978642
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1983 Publications
Monnat, R.J., Jr. and Loeb, L.A. (1983) Mechanisms of neoplastic transformation:"Molecular Biology and Malignancy" Series Introduction). Cancer Invest. 1:175-183.
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1982 Publications
Rutledge, J.D., Haas, J.E., Monnat, R.J., Jr. and Milstein, J.M. (1982) Hypertrophic cardiomyopathy is a component of subacute necrotizing encephalomyelopathy. J.Pediatr. 101:706-710. PMID: 6890100
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1980 Publications
Monnat, R.J., Jr. (1980) Gneuropeptides. New Scientist 87:54 (letter).
Monnat, R.J., Jr. (1980) Pulmonary calcification in a renal transplant recipient. Amer. Rev. Resp. Dis. 122:355 (letter).
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1979 Publications
Martin, G.M., Ogburn, C.E., Colgin, L.M., Gown, A.M., Edland, S.D. and Monnat, R.J., Jr. (1996) Somatic mutations are frequent and increase with age in human kidney epithelial cells. Human Molec. Genetics 5:215-221. doi: 10.1093/hmg/5.2.215. PMID: 8824877
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2020 Publications
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Horowitz L. F., Rodriguez A.D., Dereli-Korkut Z., Lin R., Castro K., Mikheev A., Monnat R.J. Jr., Folch A. and Rostomily R.C. (2020) Multiplexed drug testing of tumor slices using a microfluidic platform NPJ Precision Oncology 19;4:12. PMID: 32435696 PMC7237421. doi: 10.1038/s41698-020-0117-y.
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Rodriguez A.D., Horowitz L. F., Castro K., Kenerson H., Bhattacharjee N., Gandhe G., Raman A., Monnat R. J. Jr., Yueng R., Rostomily R.C. and Folch A. (2020) A microfluidic platform for fuctional testing of cancer drugs on intact tumor slices. Lab on a Chip 7;20(9):1658-1675. PMID: 32270149. doi: 10.1039/c9lc00811j
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Hasle N, Cooke A, Srivatsan S, Huang H, Stephany JJ, Krieger Z, Jackson D, Tang Weilliang, Pendyala S, Monnat RJ Jr., Trapnell C, Hatch EM, and Fowler DM (2020). High-throughput microscope-based sorting to dissect cellular heterogeneity. Molecular Systems Biology 16(6):e9442. PMID: 32500953 PMCID: PMC7273721 DOI: 10.15252/msb.20209442.
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Carbone M, Arron ST, Beutler B, Bononi A, Cavenee W, Cleaver JE, Croce CM, D'Andrea A, Foulkes WD, Gaudino G, Groden JL, Henske EP, Hickson ID, Hwang PM, Kolodner RD, Mak TW, Malkin D, Monnat RJ Jr, Novelli F, Pass HI, Petrini JH, Schmidt LS, Yang H. (2020) Tumour predisposition and cancer syndromes as models to study gene-environment interactions. Nature Reviews Cancer PMID: 32472073 DOI: 10.1038/s41568-020-0265-y.
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